结核杆菌的肝素结合性血凝素对于肺外传播是必需的
1Unité INSERM U447, Institut Pasteur de Lille, 1 rue du Prof. Calmette, F-59019 Lille Cedex, France.
Nature
|July 13, 2001
概括
肝素结合性血凝素粘附素 (HBHA) 对Mycobacterium结核病传播到肺以外的器官至关重要. 针对HBHA的抗体可能会增强对结核病的免疫保护.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 病变的发生和发病.
背景情况:
- 结核病 (TB) 是全球主要的传染病死亡原因,由结核菌菌引起.
- 结核病感染通常始于肺部,并可能扩散到其他组织.
- 虽然已知毒性因子,但没有哪一个与肺外结核传播有具体联系.
研究的目的:
- 为了确定参与Mycobacterium结核病的肺外传播的毒性因素.
- 为了研究肝素结合性血凝素粘合素 (HBHA) 在菌根菌病原发生中的作用.
主要方法:
- 在M.结核病和Mycobacterium bovis Bacille Calmette-Guérin (BCG) 中hbhA基因的基因破坏.
- 评估突变菌株与上皮细胞和巨细胞类细胞的相互作用.
- 在注射突变菌株后,评估小鼠的脏和肺部殖民.
- 测试抗HBHA抗体对真菌细菌传播的影响.
主要成果:
- 破坏hbhA基因会影响菌根菌与上皮细胞的相互作用,但不会影响巨细胞.
- 在小鼠内鼻感染后,hbhA突变菌株显示大大减少了脏殖民.
- 用抗HBHA抗体覆盖野生型M.结核病减少了传播.
结论:
- 肝素结合性血凝素粘附素 (HBHA) 在M.结核病的肺外传播中起着至关重要的作用.
- 与非食细胞细胞的相互作用,由HBHA等粘附蛋白促进,在结核病发病过程中很重要.
- 针对HBHA的抗体反应可能有助于对结核病的保护性免疫力.
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