Rab23是鼠标Sonic hedgehog信号通路的一个基本负调节器
J T Eggenschwiler1, E Espinoza, K V Anderson
1Molecular Biology Program, Sloan-Kettering Institute, New York, New York 10021, USA.
Nature
|July 13, 2001
概括
鼠标开放大脑 (opb) 基因Rab23在Sonic hedgehog (Shh) 下游作用,调节脊髓发育. 这揭示了神经管模式中的Shh独立机制.
科学领域:
- 发展生物学 发展生物学
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
背景情况:
- 鼠标开放大脑 (opb) 和Sonic hedgehog (Shh) 基因在脊髓神经模式中起着相反的作用.
- opb对于背部细胞类型至关重要,而Shh对于腹部细胞类型至关重要.
研究的目的:
- 为了研究神经模式中opb和Shh之间的关系.
- 为了确定opb基因的分子身份和功能.
主要方法:
- 基于地图的克隆来识别OPB基因.
- 对Shh和Shh opb双突变小鼠进行分析,以研究神经模式.
- 研究Rab23在Shh信号通路中的作用.
主要成果:
- 该opb基因编码Rab23,一种囊泡运输蛋白.
- 在脊髓发育中,opb 作用于 Shh 的下游.
- 包括地板板在内的腹腔细胞类型存在于Sh opb双突变体中,表明Shh独立的模式机制.
- 背部化信号激活Rab23转录以抑制背部神经细胞中的Shh通路.
结论:
- Rab23 (opb) 在Shh的下游发挥作用,调节腹部神经细胞命运.
- 独立于SH的机制有助于神经管的背腔腹腔模式.
- 通过Rab23.3.通过dorsalising信号来调节Shh通路.
相关概念视频
01:14Rab Proteins
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:25Rab Cascades
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...