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红红素耐药性与A组链球菌进入人类呼吸道细胞的能力之间的关联
B Facinelli1, C Spinaci, G Magi
1Institute of Microbiology, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131, Ancona, Italy. b.facinelli@popsci.unian.it
Lancet (London, England)
|July 17, 2001
概括
抗红素的A型链球菌越来越多地与增强的细胞入侵有关. 这种组合可能使细菌能够逃避抗生素治疗,如宏化物和β-乳酸盐.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 在欧洲,A型链球菌 (GAS) 对红红素的耐药性正在上升.
- 气体可以侵入并存活在人类呼吸道细胞内,这是以前没有强调的特征.
- prtF1基因对于GAS进入上皮细胞至关重要,而类抗生素会透宿主细胞,与β-乳糖类不同.
研究的目的:
- 为了调查prtF1基因的存在,并评估意大利A组链球菌分离体中的细胞入侵效率.
- 确定红红素耐药性和GAS入侵人类呼吸道细胞的能力之间的关联.
主要方法:
- 在意大利检查了74个耐红素和52个敏感的GAS分离物,这些分离物来自患有喉炎的儿童.
- 使用三盘试验和PCR评估了红红素耐药性表型和耐药性决定因素 (ermB, ermTR, mefA).
- 通过PCR研究了prtF1基因的存在,并评估了在培养的人类呼吸道细胞中所有菌株的细胞入侵效率.
主要成果:
- 与易感菌株 (21%) 相比,显著更高比例的耐红素菌株是prtF1阳性 (89%).
- 高度侵入细胞的分离物 (>10%的侵入效率) 在抗红红素菌株 (80%) 中明显比敏感菌株 (10%) 更普遍.
- 所有缺乏prtF1的抗红红素菌株都表现出M耐药表型.
结论:
- 红红素耐药性和A组链球菌中细胞侵入性增加之间存在一个意想不到的联系.
- 具有红红素耐药性和细胞内侵入能力的GAS菌株引起了重大的临床关注.
- 这些具有双重特征的菌株可能因耐药性而逃避用宏类药物治疗,而由于细胞内位置而逃避β-乳糖类药物治疗.
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