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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jun 29, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

一种离子液体形式的DNA:核酸的氧化还原活性盐.

A M Leone1, S C Weatherly, M E Williams

  • 1Department of Chemistry, Venable and Kenan Laboratories, University of North Carolina, Chapel Hill, North Carolina 27599-3290, USA.

Journal of the American Chemical Society
|July 18, 2001
PubMed
概括
此摘要是机器生成的。

新的离子液体利用双重DNA作为阴离子和金属复合物作为阴离子. 这些DNA融盐使电化学询问和选择性瓜氧化成为可能,提供基于核酸序列的调节性质.

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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

相关实验视频

Last Updated: Jun 29, 2026

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

科学领域:

  • 电化学 电化学 电化学
  • 材料科学 材料科学 材料科学
  • 生物化学 生物化学

背景情况:

  • 离子液体具有独特的溶剂性能.
  • 过渡金属复合物可以用聚乙烯功能化.
  • 可以将DNA整合到新的材料架构中.

研究的目的:

  • 合成和表征新型离子液体,包括双重DNA和聚乙烯装饰过渡金属复合体.
  • 为了研究这些DNA融盐的电化学特性和扩散行为.
  • 探索这些材料在选择性DNA修饰和传感方面的潜力.

主要方法:

  • 聚乙烯装饰过渡金属复合物的合成 (Fe,Co).
  • 形成基于DNA的离子液体 (盐).
  • 使用微电极 (循环电压测量) 的电化学询问.

主要成果:

  • 由于离子扩散,DNA盐会呈现周期性伏特ammograms.
  • 哥巴尔特复合体显示了Co(III/II) 氧化还原对,其扩散系数受DNA粘度的影响.
  • 一种混合Co和Fe复合物的混合物证明了由电生成Fe(III) 氧化瓜的催化波.

结论:

  • 溶解的DNA盐代表了一类新的功能性材料.
  • 材料属性可以通过核酸序列调整.
  • 这些材料可以直接用电化学探究,并用于选择性DNA氧化.