使用残余二极合物的灵活寡糖的形态分析
F Tian1, H M Al-Hashimi, J L Craighead
1Contribution from the Complex Carbohydrate Research Center, The University of Georgia, 220 Riverbend Rd., Athens, Georgia 30602-4712, USA.
Journal of the American Chemical Society
|July 18, 2001
概括
我们开发了一种新的NMR方法来分析寡糖的运动. 这种技术在甲基3,6-di-O- ((alpha-D-mannopyranosyl) -alpha-D-mannopyranoside中的α (1,3) 链路上显示出有限的运动和α (1,6) 链路上的显著灵活性.
科学领域:
- 碳水化合物化学 碳水化合物化学
- 生物物理化学 生物物理化学
- 结构生物学 结构生物学
背景情况:
- 寡糖在生物过程中起着至关重要的作用.
- 了解它们的形状和运动特性对于阐明它们的功能至关重要.
- 传统的NMR方法对于分析这些动态可能是复杂的.
研究的目的:
- 提出一种新的,简化的方法来分析寡糖的动态.
- 为了研究甲基3,6-di-O-(alpha-D-mannopyranosyl) -alpha-D-mannopyranoside的构造和运动特性.
- 为了比较不同糖酸结合的内部运动.
主要方法:
- 在溶液中的残余二极合物的顺序矩阵分析.
- 各种残余二极合的组合: (1) D CH, (2) D CH,和 D H H H).
- 使用两种不同的定向介质 (双胞胎和菌体) 解决trimannoside单个环的顺序矩阵.
主要成果:
- 顺序参数表示在α (1,3) 连接处的有限内部运动.
- 在α (1,6) 连接处观察到显著的内部运动.
- 使用不同的定向介质确定的两个不同的结构证实了alpha (1,3) 的有限运动和alpha (1,6) 的大的运动幅度.
结论:
- 新的NMR方法简化了数据采集和分析.
- 在α (1,3) 连接处的内部运动是最小的,表明一个稳定的首选形状.
- 阿尔法 (1,6) 连接表现出相当大的灵活性,形状最好被描述为虚拟形态.
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