指核的活性:分析蛋白质包装和静电选

A T Maynard1, D G Covell

  • 1Frederick Cancer Research and Development Center, Screening Technologies Branch, DCTD, NCI, NIH, SAIC, Frederick, Maryland 21702, USA. covell@faxpdc.ncifcrf.gov

概括

指进化为屏蔽反应性硫酸盐,其稳定性因蛋白质环境而异. 不稳定的指,就像在人类雌激素受体中一样,可能会被氧化还原调节,影响转录和癌症进展.

相关概念视频

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...