目标模拟印制聚合物与叶酸和相关化合物的亲和力
M Quaglia1, K Chenon, A J Hall
1Contribution from the Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg Universität Mainz, Duesbergweg 10-14, D-55128 Mainz, Germany.
Journal of the American Chemical Society
|July 18, 2001
概括
分子印记聚合物 (MIPs) 被合成用于叶酸识别. 使用三甲作为模板的聚合物与甲基酸在HPLC分析中表现出对丁衍生物的优越选择性.
科学领域:
- 聚合物化学 聚合物化学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 开发用于叶酸和相关的丁酸衍生物的选择性材料对于各种分析和制药应用至关重要.
- 分子印记聚合物 (MIP) 提供了一种有前途的方法,用于创建具有高特异性的合成受体.
研究的目的:
- 为了比较两种不同的合成策略,以创建与叶酸和替代性丁酸相亲密的MIP.
- 评估使用不同功能单体和模板分子进行选择性结合和分离合成的MIP的性能.
主要方法:
- 使用模板 (甲基酸) 在批量和色谱模式下选功能性单体 (例如,2-乙烯基二,甲酸).
- 使用有机可溶二叶酸减少酶抑制剂作为模板与甲基酸合成MIPs.
- 高性能液态染色学 (HPLC) 分析以评估合成的MIP的选择性和保留性.
- 用Scatchard分析量化结合部位的特征.
主要成果:
- 基于2-乙烯二的MIP显示选择性和保留性不足.
- 用甲基酸和基于瑞丁的模板合成的MIP表现出对替代瑞丁的增强选择性.
- 印有三甲的聚合物在有机移动相中表现出最高的选择性,观察到双相结合行为.
- 模板基本性与甲基烯酸和色谱选择性的关联强度相关.
结论:
- 甲基酸作为一个功能性单体,结合适当的基于丁的模板,如trimethoprim,为叶酸类似物产生高度选择性的MIP.
- 模板的选择显著影响了MIP的保留率和选择性,特别是在有机移动阶段.
- 这些MIP显示了需要选择性认可替代丁的应用的潜力.
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