含有乳酸桥接侧链的富含Aib的,作为3(10) -螺旋的模型
E Schievano1, A Bisello, M Chorev
1University of Padova, Department of Organic Chemistry, Biopolymer Research Center, C.N.R, Via Marzolo 1, 35131 Padua, Italy.
Journal of the American Chemical Society
|July 18, 2001
概括
富含α-aminoisobutyric acid (Aib) 的乳酸桥接寡合体被合成为模型的3(10) -螺旋. 这些富含Aib的酸体显示3(10) -螺旋形成增加与链延长,通过NMR和模拟证实.
科学领域:
- 类化学 类化学
- 结构生物学 结构生物学
- 生物物理化学 生物物理化学
背景情况:
- 3(10) - 螺旋是蛋白质中常见的二次结构图案.
- 设计偏好采用特定螺旋结构的质对于理解蛋白质折叠和开发基于的治疗方法至关重要.
- 已知α-aminoisobutyric酸 (Aib) 残留物可以促进螺旋形状.
研究的目的:
- 设计和合成新的乳酸桥接寡合体作为3(10) -螺旋的模型.
- 研究这些在溶液中的结构偏好.
- 为了探索链条延长对3(10) -螺旋形成的影响.
主要方法:
- 合成富含Aib,侧链乳酸桥接的寡合体 (n=1,2,3).
- 使用循环二重化 (CD) 和核磁共振 (NMR) 光谱学在水性SDS微粒中的形态表征.
- 计算分析包括分子动力学模拟和NOE (核过量效应) 连接性分析.
主要成果:
- 核磁共振 (NMR) 数据显示,3(10) -螺旋体的形成增加,寡合体长度增加 (二元和三元).
- 胺质子的NOE连接性和温度系数支持一个稳定的,与结合的螺旋结构.
- 分子动力学模拟和键分析证实了C=O(i) - - -HN(i+3) 相互作用的普遍性,这是3(10) -螺旋的特征.
- CD光谱为这些的3(10) 螺旋形状提供了光谱签名.
结论:
- 在富含Aib的寡合体中侧链乳酸桥接有效地增强了对3(10) - 螺旋的构造偏好.
- 链条的延长显著促进了稳定的3(10) 螺旋结构的形成.
- 这些模型提供了对3(10) -螺旋体的光谱和结构特征有价值的见解.
相关概念视频
Molecular Models
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Newman Projections
Different notations are used to represent the three-dimensional structure of molecules on two-dimensional surfaces. One of the most commonly used representations is the dash-wedge formula. The dashed wedges, solid wedges, and the plane lines indicate the groups situated behind the plane, coming out of the plane, and in the plane, respectively.
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as conformers.
The organic molecules rotate across the single bonds leading to numerous temporary three-dimensional structures of varying energy known as conformers.
Generation of Straight or Branched Actin Filaments
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Multi-pass Transmembrane Proteins and β-barrels
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as G-protein-linked receptors (GPCRs) and...


