在有机溶剂中氧化谷氨的自组装和凝
1Department of Medicinal Chemistry, Box 357610, University of Washington, Seattle, Washington 98195-7610, USA.
Journal of the American Chemical Society
|July 18, 2001
概括
氧化谷氨 (GSSG) 自组装成粉样纤维,在有机溶剂中形成凝. 这种特定的自我识别表明了蛋白质聚合和折叠的潜在作用.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 分子生物学分子生物学
背景情况:
- 谷氨 (gamma-glu-cys-gly) 是一种重要的三抗氧化剂.
- 氧化二硫化物形式,GSSG,因其自组装特性而受到研究.
研究的目的:
- 为了研究GSSG在各种溶剂中的凝行为.
- 为了描述GSSG诱导的凝的结构性质.
- 为了探索GSSG自组装的特点.
主要方法:
- 在DMSO,DMF和甲醇水溶液中的凝实验.
- 刚果红结合测定和双断度测量.
- 传输电子显微镜 (TEM) 用于结构分析.
- 1H 核磁共振和循环二元化 (CD) 光谱学.
主要成果:
- 在低度 (1.5 mM) 时,GSSG会形成透明的,热可逆的凝.
- GSSG凝表现出类似粉样蛋白的特征,包括刚果红色结合和双折断.
- TEM揭示了一个直径约75纳米的纤维状网络.
- 相关酸没有形成凝,表明高特异性.
- 核磁共振和CD数据表明GSSG的溶剂依赖的结构变化.
结论:
- 在有机溶剂中,GSSG自组装成类似β片的结构,导致凝.
- 马-氨基胺结合对GSSG的凝行为至关重要.
- GSSG的自我识别可能对生物系统中的蛋白质聚合和硫化-硫化交换产生影响.
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