Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
Other Nuclides: 31P, 19F, 15N NMR01:16

Other Nuclides: 31P, 19F, 15N NMR

Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a high...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Scabies in monasteries in Phnom Penh, Cambodia.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

RIDME spectroscopy on high-spin Mn<sup>2+</sup> centers.

Physical chemistry chemical physics : PCCP·2016
Same author

The effect of gamma-ray irradiation on the Mn(II) speciation in Deinococcus radiodurans and the potential role of Mn(II)-orthophosphates.

Metallomics : integrated biometal science·2015
Same author

Variations in Mn(II) speciation among organisms: what makes D. radiodurans different.

Metallomics : integrated biometal science·2014
Same author

Impact of Proximal and Distal Pocket Site-Directed Mutations on the Ferric/Ferrous Heme Redox Potential of Yeast Cytochrome-<i>c</i>-Peroxidase.

Theoretical chemistry accounts·2013
Same author

Herbicide-induced changes in charge recombination and redox potential of Q(A) in the T4 mutant of Blastochloris viridis.

Biochemistry·2005

相关实验视频

Updated: Jun 24, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

在野生类型和 W191G 突变细胞染色体c 过氧化酶的 W191G 突变体中对三法尼尔和铁基中间体进行了多频高场 EPR 研究.

A Ivancich1, P Dorlet, D B Goodin

  • 1Section de Bioénergétique, URA 2096 CNRS, Département de Biologie Cellulaire et Moléculaire, CEA Saclay, 91191 Gif-sur-Yvette, France.

Journal of the American Chemical Society
|July 18, 2001
PubMed
概括

高场电子磁共振 (EPR) 光谱精确地描述了细胞染色体c过氧化酶 (CcP) 中的中间激素. 这种技术识别了短暂的铁基,并提供了对它们的电阳性环境的洞察.

更多相关视频

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

相关实验视频

Last Updated: Jun 24, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
13:21

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Published on: August 18, 2012

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
06:01

EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1

Published on: November 26, 2014

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans
12:57

Investigating the Spreading and Toxicity of Prion-like Proteins Using the Metazoan Model Organism C. elegans

Published on: January 8, 2015

科学领域:

  • 生物物理化学 生物物理化学
  • 频谱学是一种光谱学.
  • 酶学 是一种酶学.

背景情况:

  • 细胞染色体c过氧化酶 (CcP) 在细胞防御机制中起着至关重要的作用.
  • 了解CCP的中间激素是阐明其催化机制的关键.
  • 之前的研究使用了较低频率的EPR,限制了过渡物种的解决.

研究的目的:

  • 用多频高场EPR来表征野生类型和突变CcP中的基质中间体.
  • 为了分析CcP化合物I中的oxoferryl-tryptophanyl基因对.
  • 检测和表征在CCP催化过程中形成的过渡性铁基.

主要方法:

  • 多频 (95,190,285 GHz) 高场电子偏磁共振 (EPR) 光谱.
  • 使用自旋哈密尔顿式与异型自旋-自旋相互作用对EPR光谱的分析.
  • 为准确的光谱模拟提供异型汉密尔顿式的数值解.

主要成果:

  • 使用单一的旋转参数集,精确模拟CcP化合物I中介光谱在广泛的频率范围内 (9-285 GHz).
  • 在野生型CcP中明确检测了一种短暂的铁基,在添加H2O2后60秒出现,在1小时后消失.
  • 在W191G CcP突变中,基质中间体的表征,g值与短暂的铁基和玛辐射的铁酸相似,表明电阳性环境.

结论:

  • 高场EPR提供了卓越的分辨率,用于表征CcP中的过渡激素中间体.
  • 该研究证实了CcP化合物I中间体的结构,并确定了一种短暂的铁基.
  • 已识别的铁基存在于CCP内的电阳性和分布式静电环境中.