介质变化过程中非交叉和交叉重组的不同时间和控制
1Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Cell
|July 20, 2001
概括
在酵母中, meiotic 重组显示了两个不同的途径. 非交叉产品在假日交叉点早期形成,而交叉产品则在交叉点解决后出现,这表明不同的监管机制.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 介质重组对于遗传多样性至关重要.
- 单元模型提出霍利代交叉口作为交叉和非交叉产品的中心中间体.
- 预计这两种产品类型都含有异重复DNA.
研究的目的:
- 调查非交叉产品和交叉产品在变过程中的时间和调节.
- 挑战介质性重组的单元模型.
- 阐明Saccharomyces cerevisiae中中性重组的独特途径.
主要方法:
- 对Saccharomyces cerevisiae中介性重组中间体和产品的分析.
- 对ndt80突变的研究,以评估霍莱德结分辨率在重组途径中的作用.
- 时间分析异质双重DNA的形成和霍莱德结的分辨率.
主要成果:
- 非交叉的异质双重产品与霍莱德连接中间体同时形成.
- 交叉产品是在稍后生成的,在Holliday连接中间体的分辨后.
- 非交叉和交叉重组途径受到不同的调节,如在ndt80突变体中所示.
结论:
- 交叉形成取决于霍莱德交叉点中间体的分辨率.
- 很大一部分非交叉复合物是通过另一个更早的途径生成的.
- 介质性重组至少涉及两个不同的途径,具有差异调节.
相关概念视频
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The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
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Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
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Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
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Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
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Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...


