通过直接合的同位素和遗传学分析揭示了消耗甲的古生物
V J Orphan1, C H House, K U Hinrichs
1Monterey Bay Aquarium Research Institute, Moss Landing, CA 95039, USA.
概括
海洋微生物消耗了大部分海洋甲. 特定的古生物 (Methanosarcinales) 吸收轻甲,而其他生物则使用不同的碳来源,揭示了沉积物中的微生物代谢活动.
科学领域:
- 微生物学 微生物学
- 地质化学 地质化学
- 生物地质化学生物地质化学
背景情况:
- 无氧海洋沉积物是全球甲循环至关重要的微生物群落的宿主.
- 超过80%的海洋甲是由这些环境中的微生物消耗的.
- 在富含甲的沉积物中,代谢上相互依赖的微生物群体很常见.
研究的目的:
- 研究无氧海洋沉积物中的微生物的代谢活动和身份.
- 为了确定这些沉积物中不同微生物群体使用的碳来源.
- 展示一种同时识别和评估微生物代谢功能的方法.
主要方法:
- 光在位杂交 (FISH) 用于微生物识别.
- 二次离子质谱 (SIMS) 用于稳定同位素分析 (13C/12C比).
- 结合FISH-SIMS将微生物身份与代谢活动联系起来.
主要成果:
- 特定的古老细胞,与Methanosarcinales关联,显示13C显著耗尽 (每千分之-96).
- 这种13C耗尽表明这些古物质对同位素轻甲进行了同化.
- 其他微生物物种的13C/12C比率更高,这表明使用替代碳来源.
结论:
- 该研究成功地确定了在海洋沉积物中同化甲的特定考古群体.
- 在无氧环境中的微生物联盟中存在不同的碳同化途径.
- 结合FISH-SIMS方法是有效的同时确定微生物的身份和代谢活动在现场.
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