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相关概念视频

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Mechanical Protein Function01:58

Mechanical Protein Function

Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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相关实验视频

Updated: Jun 21, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

使用蛋白质芯片对蛋白质活动的全球分析.

H Zhu1, M Bilgin, R Bangham

  • 1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.

Science (New York, N.Y.)
|July 28, 2001
PubMed
概括
此摘要是机器生成的。

研究人员创建了一个酵母蛋白质微阵列来研究蛋白质相互作用. 这种工具确定了新的calmodulin和脂结合蛋白,为药物查和理解蛋白质修饰铺平了道路.

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

相关实验视频

Last Updated: Jun 21, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

科学领域:

  • 蛋白质组学和系统生物学
  • 生物化学和分子生物学

背景情况:

  • 了解酵母蛋白质组对于破译真核细胞细胞功能至关重要.
  • 需要高通量方法来分析大量的酵母蛋白及其相互作用.

研究的目的:

  • 开发一个全面的酵母蛋白质组微阵列,用于大规模的生化查.
  • 在酵母蛋白质组中识别新的蛋白质-蛋白质和蛋白质-脂相互作用.
  • 探索蛋白质组微阵列在药物发现和翻译后修改分析中的实用性.

主要方法:

  • 克隆和过度表达的5800酵母开放的阅读框架.
  • 净化相应的蛋白质并将其打印到幻灯片上,以创建高密度蛋白质组微阵列.
  • 选微阵列与calmodulin和脂的相互作用.

主要成果:

  • 一个酵母蛋白质微阵列的成功构建和选.
  • 识别了众多新型的卡尔莫杜林结合蛋白和脂相互作用蛋白.
  • 在许多卡尔莫杜林结合蛋白中发现了一种共同的结合基因.

结论:

  • 蛋白质组微阵列是一种可行的和强大的工具,用于全面选真核蛋白质组.
  • 这种方法有助于发现新的蛋白相互作用和生物化学活动.
  • 酵母蛋白质微阵列有可能用于药物相互作用查和检测翻译后修改.