Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbes in Food Production01:29

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Prompting large language models and evaluating inter- and intra-rater agreement for cancer progression assessment from radiology reports.

ESMO real world data and digital oncology·2026
Same author

Parent-Reported Health and Neurodevelopment in Children Who Were Mechanically Ventilated in Infancy.

Acta paediatrica (Oslo, Norway : 1992)·2025
Same author

Lateral gain is impaired in macular degeneration and can be targeted to restore vision in mice.

Nature communications·2022
Same author

Synthetic Biology of Cannabinoids and Cannabinoid Glucosides in <i>Nicotiana benthamiana</i> and <i>Saccharomyces cerevisiae</i>.

Journal of natural products·2020
Same author

Time trends and patient selection in the use of continuous electrocardiography for detecting atrial fibrillation after stroke: a nationwide cohort study.

European journal of neurology·2020
Same author

Gastrointestinal sequelae and growth impairment at school age following necrotising enterocolitis in the newborn period.

Acta paediatrica (Oslo, Norway : 1992)·2019

相关实验视频

Updated: Jun 28, 2026

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example
06:52

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example

Published on: June 19, 2011

通过工程原糖化物合成对草食动物的抵抗力.

D B Tattersall1, S Bak, P R Jones

  • 1Plant Biochemistry Laboratory, Department of Plant Biology, Centre for Molecular Plant Physiology, Department of Chemistry, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871, Frederiksberg C, Denmark.

Science (New York, N.Y.)
|July 28, 2001
PubMed
概括
此摘要是机器生成的。

基因工程植物现在可以产生dhurrin,一种天然化合物. 在Arabidopsis thaliana中这种dhurrin生产提供了对跳甲虫的抵抗力,展示了其在植物防御策略方面的潜力.

更多相关视频

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera
04:51

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera

Published on: May 26, 2023

相关实验视频

Last Updated: Jun 28, 2026

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example
06:52

Characterizing Herbivore Resistance Mechanisms: Spittlebugs on Brachiaria spp. as an Example

Published on: June 19, 2011

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera
04:51

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera

Published on: May 26, 2023

科学领域:

  • 植物分子生物学 植物分子生物学
  • 生物化学 生物化学
  • 化学生态化学生态学

背景情况:

  • 原性葡萄糖酸是植物防御化合物.
  • 杜林是一种由氨酸衍生的原性葡萄糖体,在 Sorghum bicolor 中发现.
  • 了解杜林合成的遗传基础对于其应用至关重要.

研究的目的:

  • 为了将杜林合成途径从 Sorghum bicolor 转移到 Arabidopsis thaliana.
  • 评估转基因阿拉比多普西斯 (Arabidopsis) 中的杜林的生产和储存情况.
  • 评估dhurrin在赋予植物对草食动物的防御方面的有效性.

主要方法:

  • 基因工程技术被用来引入dhurrin生物合成基因进入Arabidopsis thaliana.
  • 进行了代谢分析以确认杜林的产生和积累.
  • 使用跳甲虫Phyllotreta nemorum进行了生物测试,以评估植物的耐药性.

主要成果:

  • 在转基因Arabidopsis thaliana中,完整的杜林合成途径已成功重建.
  • 转基因植物积累了大量的dhurrin.
  • 阿拉比多普西斯·塔利亚纳表达杜林的植物表现出对Phyllotreta nemorum草食菌的增强抵抗力.

结论:

  • 杜林通路的转移是可行的,并导致在异质植物系统中产生功能性化合物.
  • 杜林赋予了Phyllotreta nemorum的显著抵抗力,验证了它在植物防御中的作用.
  • 这项研究突出了利用原糖化物在作物保护和工程方面的潜力.