鉴定了一种与Heliothis virescens中Bt耐药性相关的基因
L J Gahan1, F Gould, D G Heckel
1Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA.
Science (New York, N.Y.)
|August 4, 2001
概括
科学家们确定了棉花害虫Heliothis virescens的遗传突变,导致对Bacillus thuringiensis (Bt) 作物的耐药性. 这一发现允许对耐药昆虫进行DNA选,有助于害虫控制策略.
科学领域:
- 农业昆虫学 农业昆虫学
- 分子遗传学 分子遗传学
- 虫害管理 虫害管理 虫害管理
背景情况:
- 生产Bacillus thuringiensis (Bt) 毒素的转基因作物对于控制昆虫害虫至关重要.
- 昆虫对Bt毒素耐药性的基因机制尚不清楚.
研究的目的:
- 为了阐明棉花害虫Heliothis virescens对Bt毒素Cry1Ac的耐药性的分子基础.
- 开发一种早期检测Bt耐药性演变的方法.
主要方法:
- 研究了Heliothis virescens中高水平Cry1Ac耐药性的遗传基础.
- 鉴定了一种以逆转移素为媒介的插入,破坏了卡德林超级家族基因.
主要成果:
- 一种特定的卡德林超级家族基因破坏与高水平的Cry1Ac耐药性直接相关.
- 这种基因变异赋予了衰退性耐药性,使得现场监测具有挑战性.
- 鉴定的突变允许直接基于DNA对抗性等位基因进行查.
结论:
- 干林超级家族基因的破坏是Heliothis virescens中Bt耐药性的关键机制.
- 现在可以有效地对抗性异构体进行DNA选.
- 这有助于在农业环境中主动管理Bt耐药性.
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