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相关概念视频

Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

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相关实验视频

Updated: Jun 21, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

RNA:指导基因沉默的指导基因

M Matzke1, A J Matzke, J M Kooter

  • 1Institute of Molecular Biology, Austrian Academy of Sciences, A-5020 Salzburg, Austria. mmatzke@imb.oeaw.ac.at

Science (New York, N.Y.)
|August 11, 2001
PubMed
概括

来自双链RNA的小RNA在各种生物体中触发基因沉默. 这种RNA沉默机制影响植物和动物的基因表达后转录和表观遗传.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 小RNAs是基因表达的关键调节者.
  • RNA沉默是一种在各种生物体中保存的生物过程.
  • 双链RNA (dsRNA) 是小型RNA生成的前体.

研究的目的:

  • 阐明小RNA介导基因沉默的机制.
  • 突出RNA沉默在表观遗传调节中的作用.
  • 探索RNA沉默在发育中的重要性.

主要方法:

  • 从dSRNA裂变中对小RNA生物发生的分析.
  • 研究细胞质和基因组基因沉默通路.
  • 通过mRNA降解研究转录后基因沉默.
  • 在植物中通过DNA甲基化检查转录基因沉默.

主要成果:

  • 小RNA诱导细胞质和基因组中的表观遗传基因沉默.
  • 小RNAs指导补充信使RNAs的降解.
  • 植物利用RNA沉默来通过DNA甲基化进行转录基因沉默.

更多相关视频

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
09:18

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

Published on: June 2, 2016

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

相关实验视频

Last Updated: Jun 21, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
08:25

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

Published on: March 20, 2016

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
09:18

TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

Published on: June 2, 2016

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

结论:

  • RNA沉默是对外来序列的强有力的防御.
  • 小RNA通路在植物和动物发育中起着至关重要的作用.
  • 表观遗传修饰是RNA介导的基因调节的核心.