Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Physical Properties of Amines01:26

Physical Properties of Amines

3.2K
Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
3.2K
Types of Toxins01:36

Types of Toxins

3.7K
Humans continually engage with an environment rich in potentially harmful chemicals. These are introduced to our bodies through inhalation, ingestion, or skin contact. These chemicals exist in various forms, such as air and environmental pollutants, agricultural chemicals, organic solvents, and heavy metals.
Air pollutants, primarily gases, pose significant threats to respiratory health, leading to conditions like hypoxia, lung cancer, and in extreme cases, death.
Environmental pollutants like...
3.7K
Prevention of Further Absorption of Poison01:14

Prevention of Further Absorption of Poison

1.4K
In cases of acute poisoning, the primary objective is to prevent further absorption of the toxic substance into the body. Immediate interventions using various decontamination techniques targeting the gastrointestinal (GI) tract can achieve this. Decontamination is crucial to prevent poison from entering the systemic circulation, which involves washing affected areas with water and mild soap and removing contaminated clothing. Once external decontamination is done, attention must be turned to...
1.4K
Enhanced Elimination of Poison01:26

Enhanced Elimination of Poison

1.1K
Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...
1.1K
Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

1.6K
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
1.6K
Bacterial Toxins01:12

Bacterial Toxins

148
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
148

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Sexual dimorphism in campylobacteriosis.

Epidemiology and infection·2007
Same author

Re-structuring the host cell: up close with Salmonella's molecular machinery.

Microbes and infection·2004
Same author

Role of tyrosine kinases and the tyrosine phosphatase SptP in the interaction of Salmonella with host cells.

Cellular microbiology·2001
Same author

Maintenance of an unfolded polypeptide by a cognate chaperone in bacterial type III secretion.

Nature·2001
Same author

Salmonella interactions with host cells: type III secretion at work.

Annual review of cell and developmental biology·2001
Same author

Induction of specific CD8+ memory T cells and long lasting protection following immunization with Salmonella typhimurium expressing a lymphocytic choriomeningitis MHC class I-restricted epitope.

Vaccine·2001

相关实验视频

Updated: May 3, 2026

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

8.4K

细菌毒性中的结构模仿.

C E Stebbins1, J E Galán

  • 1Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA.

Nature
|August 17, 2001
PubMed
概括

微生物病原体使用功能模仿来操纵宿主细胞. 这涉及直接蛋白质同类物或结构相似的效应物,它们在演化过程中趋同,突出了多样化的分子策略.

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 微生物病原体采用复杂的策略来破坏宿主细胞功能,以造福自己.
  • 功能模仿,即病原体分子模仿宿主活动,是这一过程中的关键机制.
  • 这种模仿可以通过直接的进化关系 (同质) 或独立进化 (融合进化) 发生.

研究的目的:

  • 阐明微生物毒性因子模仿宿主功能的多种分子机制.
  • 为了区分通过同类蛋白质实现的模仿和从融合进化中产生的模仿.
  • 突出结构生物学在揭示非显而易见的分子模仿中的作用.

主要方法:

  • 对微生物毒性因子和宿主蛋白质的比较分析.
  • 生物信息学方法来识别序列同质性.
  • 结构生物学技术 (例如,X射线结晶学,冷EM) 用于确定效应蛋白结构.
  • 功能性测试用于评估效应因子对宿主细胞过程的影响.

主要成果:

  • 确定了宿主蛋白质的直接同类的毒性因子,使功能模仿成为可能.
  • 发现了缺乏序列相似性的新型病原体效应剂,通过不同的分子结构实现功能模仿.

更多相关视频

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice
07:05

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Published on: July 9, 2020

6.0K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.9K

相关实验视频

Last Updated: May 3, 2026

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
12:58

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

8.4K
A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice
07:05

A Capsule-Based Model for Immature Hard Tick Stages Infestation on Laboratory Mice

Published on: July 9, 2020

6.0K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.9K
  • 结构研究揭示了融合进化导致相似的功能模仿尽管不同的进化起源.
  • 结论:

    • 微生物病原体利用同源和融合进化的因素来模仿宿主活动的功能.
    • 结构洞察对于理解模仿机制至关重要,特别是当序列相似性缺失时.
    • 功能模仿代表了宿主-病原体相互作用中的重要和多方面的策略.