核三酸盐与RNA聚合酶的异构结合调节了转录延长的过程
J E Foster1, S F Holmes, D A Erie
1Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA.
Cell
|August 21, 2001
概括
研究人员在大肠杆菌RNA聚合酶上发现了一个新的全结合部位. 将模板核酸结合到这个部位会激活酶,增加转录延长的速度.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 酶动力学 酶动力学
背景情况:
- 转录调节涉及RNA聚合酶的结构状态.
- 控制这些状态的因素尚未完全理解.
- 了解RNA聚合酶动态对于基因表达控制至关重要.
研究的目的:
- 为了研究控制RNA聚合酶结构状态的因素.
- 阐明核酸结合调节的机制.
- 为了确定RNA聚合酶功能的全调节.
主要方法:
- 过渡状态动力学研究单核酸结合.
- 对大肠杆菌RNA聚合酶的酶动力学分析.
- 核酸结合点的表征. 核酸结合点的表征.
主要成果:
- 大肠杆菌RNA聚合酶具有与催化位点不同的全结合位点.
- 将模板核三酸盐 (NTP) 结合到全位点,可以提高核酸结合率.
- 非模拟的NTP不会结合或影响全位.
- RNA聚合酶存在于缓慢 (未激活) 和快速 (激活) 的催化状态.
- 将模板NTP绑定到全位,诱导从未激活到激活状态的过渡.
结论:
- 大肠杆菌RNA聚合酶上的一个全位调节了转录延长.
- 模板NTP与此位点结合,激活了酶,使核酸的结合速度更快.
- 这种机制提供了对转录期间RNA聚合酶结构动态的控制的洞察.
相关概念视频
Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
Bacterial Transcription
RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...


