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Updated: Jun 24, 2026

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
07:42

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 20, 2010

快速识别模块化蛋白质中中大规模的域间运动,使用二极合

D T Braddock1, M Cai, J L Baber

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases/NIH, Building 5, Room B1-301, Bethesda, MD 20892, USA.

Journal of the American Chemical Society
|August 30, 2001
PubMed
概括

No abstract available in PubMed .

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...