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Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Here, we present a method to obtain images of the intestine upon laser-induced wounding. By exposing the mouse intestine to a multiphoton laser, the loss of a single or multiple crypt(s) is induced locally. By repeatedly imaging the damaged area over months, the real-time dynamics of intestinal recovery is...

Focal Laser Ablation of Prostate Cancer: An Office Procedure

Focal Laser Ablation of Prostate Cancer: An Office Procedure

This article presents and describes an outpatient treatment for prostate cancer using focal laser ablation. Laser catheter placement is guided by MRI-ultrasound fusion imaging in a fashion similar to prostate needle biopsy. Treatment is monitored in real-time with a thermal probe, placed adjacent to the laser fiber.

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

The presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell...

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

Quantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy

During vacuum induction melting, laser-induced breakdown spectroscopy is used to perform real-time quantitative analysis of the main-ingredient elements of a molten...

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

Continuous-wave Thulium Laser for Heating Cultured Cells to Investigate Cellular Thermal Effects

An original experimental setup for heating cells in a culture dish using 1.94 µm continuous-wave laser radiation is introduced here. Using this method, the biological responses of retinal pigment epithelial (RPE) cells after different thermal exposures can be...

In vivo Laser Axotomy in C. elegans

In vivo Laser Axotomy in C. elegans

A protocol to cut neurons in C. elegans with a MicroPoint pulsed laser is presented. We describe setting up the system, immobilizing worms, and severing labeled neurons. Advantages include a relatively low-cost system and the ability to sever neuronal processes or ablate cells in...

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Updated: Jan 24, 2026

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Published on: June 9, 2023

染色素A,一个"开/关"开关,控制密核分泌颗粒生物生成.

T Kim¹, J H Tao-Cheng, L E Eiden

¹Section on Cellular Neurobiology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

|August 30, 2001

概括

染色素A (CGA) 对于密集核分泌颗粒生物发生和内分泌细胞中的激素分泌至关重要. 这种蛋白质起到关键的调节作用,驱动颗粒形成和激素储存.

科学领域:

内分泌学在内分泌学.
细胞生物学细胞生物学
神经科学是一个神经科学.

背景情况:

密集核心分泌颗粒对于内分泌细胞中受调节的激素释放至关重要.
控制这些颗粒的生物发生和功能的特定分子机制尚未完全理解.
染色素A (CGA) 是分泌颗粒的主要蛋白质成分,但其确切的作用一直在争论中.

研究的目的:

为了研究染色素A (CGA) 在密集核分泌颗粒的生物发生中的作用.
为了确定CGA是否足以驱动分泌颗粒的形成并调节激素分泌.

主要方法:

使用反感性RNA降低PC12神经内分泌细胞中CGA表达的下调.
涉及将牛CGA转化为缺乏CGA的PC12细胞的救援实验.
稳定的CGA转移到CGA缺乏的垂体细胞系6T3.3.
在非内分泌纤维细胞CV-1细胞中过度表达CGA.

主要成果:

CGA的下调导致了密核颗粒的显著损失和受损的调节分泌.
在缺陷细胞系中恢复CGA表达,挽救了受调节的分泌表型.
在非内分泌细胞中CGA的过度表达诱导了密核颗粒的形成.

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Focal Laser Ablation of Prostate Cancer: An Office Procedure

Focal Laser Ablation of Prostate Cancer: An Office Procedure

Published on: March 30, 2021

In vivo Laser Axotomy in C. elegans

In vivo Laser Axotomy in C. elegans

Published on: May 19, 2011

相关实验视频

Last Updated: Jan 24, 2026

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Laser Ablation and Intravital Microscopy to Study Intestinal Remodeling

Published on: June 9, 2023

Focal Laser Ablation of Prostate Cancer: An Office Procedure

Focal Laser Ablation of Prostate Cancer: An Office Procedure

Published on: March 30, 2021

In vivo Laser Axotomy in C. elegans

In vivo Laser Axotomy in C. elegans

Published on: May 19, 2011

结论:

染色素A (CGA) 是密集核分泌颗粒生物发生的关键决定因素.
仅CGA就足以启动分泌颗粒的形成和激素封存.
CGA作为内分泌细胞中受调节的分泌通路的关键"开/关"开关.