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Updated: Jun 21, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

Published on: September 25, 2011

通过二维免疫电泳分析进行膜蛋白分析

C A Converse, D S Papermaster

    Science (New York, N.Y.)
    |August 8, 1975
    PubMed
    概括

    一种新的技术结合了电泳和免疫电泳,用于快速分析水溶性膜蛋白. 这种方法有效地确定使用二硫酸盐 (SDS) 聚烯胺凝的蛋白质的分子量和抗原性.

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    DNA-barcode-based Multiplex Immunofluorescence Imaging to Analyze FFPE Specimens from Genetically Reprogrammed Murine Melanoma09:58

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    Published on: June 6, 2025

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    Two-dimensional Gel Electrophoresis01:22

    Two-dimensional Gel Electrophoresis

    Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
    The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
    Biological samples, such as  cells...