在重力化过程中,辅酶调节RNAs的快速再分配
1Department of Biochemistry, University of Missouri-Columbia 65211, USA.
Science (New York, N.Y.)
|January 6, 1989
概括
植物因重力而曲的重力曲,是由auxin调节的. 这项研究表明,辅酶调节的RNA分布在横向方向的20分钟内变得不对称,与曲相关.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 发育生物学是发展生物学.
背景情况:
- 重力化是植物对重力的反应,以某种方向生长.
- 辅酶是植物激素,被认为通过影响细胞延伸来调节重力.
- 奥克辛及其分子机制在重力的确切作用需要进一步阐明.
研究的目的:
- 为了研究在大豆 hypocotyls 中的重力热反应期间,辅酶调节RNA 的分布.
- 为了将动态基因表达模式与重力热曲的开始和进展相关联.
- 提供分子证据支持辅酶在调解植物重力化中的作用.
主要方法:
- 利用组织打印技术可视化RNA分布.
- 在垂直和水平方向下分析了大豆 (Glycine max) hypocotyls 中的辅酶调节RNA模式.
- 在重度刺激后,随着时间的推移监测RNA分布的变化.
主要成果:
- 在垂直导向的幼苗中,辅酶调节的RNA在 hypocotyl 的延长区域中对称分布.
- 在水平方向下,RNA分布在20分钟内变得不对称.
- 辅酶调节RNA的峰值不对称性与快速低细胞曲的开始相吻合.
结论:
- 证明了动态辅酶调节基因表达和重力的明显时间相关性.
- 提供了分子证据,证明了辅酶在植物重力化过程中的差异生长中介作用.
- 这项研究支持了这种假设,即auxin信号通路对于感知和响应引力刺激至关重要.
相关概念视频
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
02:41Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
02:53Ribosomal RNA Synthesis
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...