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Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

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Updated: Jun 14, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

通过在实验室中选择从一个包含阴离子核酸模拟物库中获得的腺酸核酸受体的定量分析.

T R Battersby1, D N Ang, P Burgstaller

  • 1Department of Chemistry, Department of Anatomy and Cell Biology, the Florida Center for Heterocyclic Compounds, University of Florida, Gainesville 32611, USA.

Journal of the American Chemical Society
|September 7, 2001
PubMed
概括

研究人员开发了新的寡核酸受体,使用具有阴离子功能组的改性核酸. 这些受体是通过体外选择和PCR放大生成的,它们进化了以合作结合两个ATP分子,显示出与自然选择的相似性.

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Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
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Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
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相关实验视频

Last Updated: Jun 14, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
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Published on: August 17, 2015

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科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 合成生物学 合成生物学

背景情况:

  • 修改后的核酸可以纳入寡核酸库.
  • 在体外选择与PCR放大相结合,可以产生功能性的核酸受体.

研究的目的:

  • 通过使用包含 cationic 核酸功能的库来研究寡核酸受体的生成.
  • 将进化受体与使用天然核酸生成的受体进行比较.

主要方法:

  • 在一个寡核酸库中,将-5-(3"-Aminopropynyl) - 2eal-deoxyuridine (dJ),一种经过修饰的核酸,纳入.
  • 使用Vent DNA聚合酶在聚合酶连锁反应 (PCR) 中扩展图书馆.
  • 在体外选择程序以分离ATP结合受体.

主要成果:

  • 选择成功地产生了能够结合ATP的功能化受体.
  • 无论是自然的还是功能化的动机都在趋同地演变,以合作地结合两个ATP分子.
  • 两种选择中的受体对ATP表现出相似的亲和力,在3mM ATP.上半和.

结论:

  • 现型的趋同表明环境因素,如亲和树脂负载,确定选择结果.
  • 在特定的选择条件下,可以获得最佳的分子表型.
  • 这项研究说明了达尔文进化论在一个自我维持的化学系统中,类似于自然选择.