抑制B. subtilis调节蛋白TRAP的TRAP抑制蛋白,AT,通过TRAP的抑制蛋白
1Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
概括
一种新发现的抗TRAP (AT) 蛋白通过抑制TRAP蛋白来发出低细胞托芬的信号,从而促进了托芬的产生. 这种机制与大肠杆菌中的机制不同.
科学领域:
- 分子生物学分子生物学
- 微生物生理学 微生物生理学
- 基因规则 基因规则
背景情况:
- 托生物合成在细菌中受到严格的调节.
- 在Bacillus subtilis中,trp操作减弱蛋白 (TRAP) 控制了酸盐的产生.
- 抗TRAP (AT) 蛋白的功能以前是未知的.
研究的目的:
- 为了阐明抗TRAP (AT) 蛋白的功能.
- 了解AT如何调节托生物合成.
- 为了比较Bacillus subtilis和Escherichia coli中托生物合成调节的情况.
主要方法:
- 研究了AT和TRAP之间的相互作用.
- 分析了AT对TRAP的RNA结合活性的影响.
- 研究了未充电的tRNA诱导AT表达的过程.
- 将AT的同质性与其他蛋白质进行比较.
主要成果:
- 在AT信号中,未充电的tRNA水平调节了托生物合成.
- AT抑制了TRAP与RNA的结合,缓解了转录抑制.
- AT表达是由未充电的tRNA诱导的.
- 酸与TRAP的托激活形式结合.
- AT与DnaJ的结域具有同质性.
结论:
- 当tRNA没有充电时,AT作为一种代谢信号来增加托生物合成.
- 在Bacillus subtilis中涉及AT的调控机制与Escherichia coli中的不同.
- 在细菌的三甲代谢途径中,AT代表了一种新的成分.
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