Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mental health impairment and recovery after a road traffic injury: where do we stand in Europe?

Frontiers in public health·2024
Same author

POP culture: the increasing perils of publish or perish. A farewell from the Editors in Chief.

Techniques in coloproctology·2024
Same author

Correction to: The impact of COVID-19 on surgical training: a systematic review.

Techniques in coloproctology·2021
Same author

Age-related changes in muscle architecture and metabolism in humans: The likely contribution of physical inactivity to age-related functional decline.

Ageing research reviews·2021
Same author

The impact of COVID-19 on surgical training: a systematic review.

Techniques in coloproctology·2021
Same author

Systematic review: the effect of right hemicolectomy for cancer on postoperative bowel function.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2020

相关实验视频

Updated: Jul 11, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

对Caenorhabditis elegans的基因表达地图

S K Kim1, J Lund, M Kiraly

  • 1Department of Developmental Biology and Genetics, Stanford University Medical School, Stanford, CA 94305, USA. kim@cmgm.stanford.edu

Science (New York, N.Y.)
|September 15, 2001
PubMed
概括

研究人员使用DNA微阵列绘制了Caenorhabditis elegans中的基因表达. 这种基因表达图有助于发现共同调节的基因和新的遗传功能,包括基因组不稳定性.

科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 发展生物学 发展生物学

背景情况:

  • 了解基因调节对于破译复杂的生物过程至关重要.
  • 凯诺哈比迪斯 (Caenorhabditis elegans) 是一个用于遗传研究的强大的模型生物.

研究的目的:

  • 开发一种用于分析大规模基因表达数据的新型可视化工具.
  • 用DNA微阵列数据识别共同调节的基因并发现新的基因功能.

主要方法:

  • 来自各种Caenorhabditis elegans实验的DNA微阵列数据的组合.
  • 根据表达特征对共同调节的基因进行分组.
  • 在3D地图中可视化基因表达相关性和密度.

主要成果:

  • 开发一个三维基因表达图的开发.
  • 证明地图在识别共同调节的基因组 (例如,热冲击,生殖系基因) 的实用性.
  • 发现潜在的新型遗传功能,例如雄性C. elegans的基因组不稳定性.

结论:

  • 3D基因表达图作为基因发现的有效工具.

更多相关视频

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Published on: October 3, 2017

Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

相关实验视频

Last Updated: Jul 11, 2026

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR
10:27

Determining Genetic Expression Profiles in C. elegans Using Microarray and Real-time PCR

Published on: July 30, 2011

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
12:15

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Published on: October 3, 2017

Hand Dissection of Caenorhabditis elegans Intestines
05:41

Hand Dissection of Caenorhabditis elegans Intestines

Published on: September 13, 2022

  • 这种方法有助于识别功能相关的基因和新的生物学见解.
  • 该研究强调了系统级分析在发现复杂遗传现象方面的潜力.