RNA结合蛋白Nrd1指导RNA聚合酶II转录的多独立的3'-末端形成
E J Steinmetz1, N K Conrad, D A Brow
1Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison, Wisconsin 53706-1532, USA.
Nature
|September 21, 2001
概括
研究人员确定了一种特定的DNA序列元素,阻止了酵母中的转录阅读. 这种元素需要RNA结合蛋白Nrd1和Nab3,以及Sen1螺旋酶才能正常工作,确保正确的基因表达.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 细胞染色体含有众多由RNA聚合酶 (pol) I,II或III转录的基因.
- 转录终止对于防止有害的多基斯特龙和反感官RNA至关重要.
- 虽然蛋白质编码基因转录的终止由pol II被理解,但非多聚烯酸转录的因素仍然未知.
研究的目的:
- 为了确定涉及到转录终止非多聚乙烯化RNAs的pol II. cis作用元素和转作用因子.
- 阐明防止酵母小核核RNA和小核RNA基因读透转录的机制.
主要方法:
- 使用酵母模型研究了转录终止.
- 确定了cis作用元素,并描述了RNA结合蛋白Nrd1和Nab3以及Sen1螺旋酶的作用.
- 评估了对pol II的C端域的要求.
主要成果:
- 发现了一种作用于 cis 的元素,可以阻止小核RNA和小核RNA基因的读透转录.
- 证明了RNA结合蛋白Nrd1可以识别这种元素.
- 显示了Nab3,Sen1和pol II C终端域对于高效的终止是必不可少的.
结论:
- 已识别的cis作用元素和相关蛋白质 (Nrd1,Nab3,Sen1) 调节特定非编码RNA的转录终止.
- 这些因素还在维持Nrd1mRNA水平方面发挥着作用,这表明在转录处理和基因表达控制方面具有更广泛的作用.
相关概念视频
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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All three eukaryotic RNAPs require specific transcription factors, of which the...
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The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...


