在需要左心室辅助器件的患者中,心肌细胞因子的定量表达和亡途径的激活
1National Heart and Lung Institute at Imperial College School of Medicine, Royal Brompton and Harefield Hospital, Harefield, Middlesex.
Circulation
|September 25, 2001
概括
需要LV辅助器件 (LVAD) 的心力衰竭患者表现出炎症性细胞因子瘤缩因子-alpha (TNF-alpha),中白素-1β (IL-1β) 和IL-6的升高. 心肌和血清中的这些分子变化可能会影响LVAD治疗的时间和结果.
科学领域:
- 心脏病学 心脏病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 在LV辅助器件 (LVAD) 植入过程中患者病情恶化的机制尚未完全理解.
- 在晚期心力衰竭中研究炎症性细胞因子和亡途径至关重要.
研究的目的:
- 为了比较需要LVAD支持的患者和接受心脏移植的患者的炎症标志物和亡途径激活.
- 为了阐明恶化的心力衰竭患者的分子差异.
主要方法:
- 来自LVAD患者和心脏移植接受者的心肌和血清样本的分析.
- 测量瘤亡因子-α (TNF-α),联素-1β (IL-1β) 和IL-6mRNA和蛋白质水平.
- 评估细胞亡路径标记物,包括卡斯帕斯和DFF-45.
主要成果:
- 患有LVAD的患者表现出心肌TNF-alpha,IL-6和IL-1βmRNA和蛋白质的升高.
- 在LVAD患者中,TNF-alpha和IL-6的血清水平显著更高.
- 在LVAD患者中,亡途径的组件,如 procaspase-9 和 cleaved caspase-9,增加了.
结论:
- 需要LVAD支持的恶化心力衰竭患者表现出心肌和血清炎症细胞因子 (TNF-alpha,IL-1beta,IL-6) 的增加.
- 在这些患者中存在亡途径的改变.
- 这些发现表明潜在的治疗影响,并影响LVAD插入的最佳时间.
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