内心肌氧化合成酶和冠状动脉内皮功能在人类心脏全移植中的表达
S M Wildhirt1, M Weis, C Schulze
1Department of Cardiac Surgery, Ludwig-Maximilians University, Munich, Germany.
Circulation
|September 25, 2001
概括
诱导性氧化合成酶 (iNOS) 与心脏移植中的微血管内皮功能障碍有关. 早期的iNOS表达和氧化的产生表明它在移植动脉样硬化发育中的作用.
科学领域:
- 心血管研究研究心血管研究
- 移植免疫学 移植免疫学
- 内皮细胞生物学 内皮细胞生物学
背景情况:
- 诱导性氧化合成酶 (iNOS) 在移植动脉样硬化中具有活性.
- 在没有先前病变的情况下,早期iNOS参与微血管内皮功能障碍是未知的.
- 在所有移植中早期iNOS的预测值是有趣的.
研究的目的:
- 在人类心脏全移植中研究iNOS mRNA表达和氧化生产.
- 评估iNOS和微血管冠状动脉内皮功能障碍之间的关联.
- 为了确定早期iNOS参与的预后价值.
主要方法:
- 研究了42名心脏移植患者在移植后的1,6和12个月.
- 使用多普勒流线评估微血管冠状动脉流速储量 (CFVR).
- 量化了iNOS mRNA,蛋白质,甲酸氨酸和跨心脏酸盐/酸盐 (NOx) 水平.
主要成果:
- 在1个月后的26.1%和12个月后的31%观察到CFVR受损.
- 患有CFVR受损的患者表现出 iNOS基因表达增加和更高的 iNOS mRNA水平.
- 在内心肌血管中存在iNOS蛋白和尼铁;在CFVR受损的人中,NOx释放量更高.
结论:
- 心脏全移植中的微血管内皮功能障碍与增加的iNOS mRNA和NOx生产有关.
- 尼铁的表达表明过氧酸盐参与了疾病过程.
- 在移植后,iNOS/氧化途径在调节微血管功能方面发挥着重要作用.
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