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人类动脉样硬化斑块中介素-18的表达和与斑块不稳定性的关系
Z Mallat1, A Corbaz, A Scoazec
1Institut National de la Santé et de la Recherche Médicale, INSERM U541, Institut Fédératif de Recherche Circulation Paris VII, Hôpital Lariboisière, Paris, France. ziad.mallat@inserm.lrb.ap-hop-paris.fr
Circulation
|October 3, 2001
概括
干白素-18 (IL-18) 在动脉样硬化斑块中高度表达,特别是在不稳定的斑块中. 这表明IL-18在斑块不稳定和急性缺血事件中起着关键作用.
科学领域:
- 心血管生物学 心血管生物学
- 免疫学 免疫学 免疫学
- 分子医学是分子医学.
背景情况:
- 互乐金-18 (IL-18) 是一种具有潜在异位性质的益炎性细胞因子.
- IL-18在动脉样硬化中的表达和特定作用在很大程度上是未知的.
- 了解IL-18的参与对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究IL-18在人动脉动脉样硬化斑块中的表达和定位.
- 为了确定IL-18表达水平和斑块不稳定性之间的关系.
- 阐明IL-18在动脉样硬化的发病过程中的潜在作用.
主要方法:
- 通过Endarterectomy获得的稳定和不稳定的人脉动脉动动脉样硬化斑块的分析.
- 利用逆转录-聚合酶连锁反应 (RT-PCR),西斑和免疫组织化学来检测IL-18.
- 使用实时定量PCR将IL-18mRNA水平与斑块不稳定性指标相关联.
主要成果:
- 与正常动脉相比,IL-18在动脉样硬化斑块中显著上调.
- IL-18主要局部化在斑块巨细胞中.
- 与无症状 (稳定) 斑块相比,在有症状 (不稳定) 的斑块中观察到较高的IL-18mRNA水平 (P<0.01).
结论:
- IL-18是动脉样硬化斑块不稳定的关键媒介.
- 增加IL-18表达与斑块不稳定性和急性缺血综合征的风险增加有关.
- 这些发现凸显了IL-18作为治疗动脉样硬化的潜在治疗点.
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