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相关概念视频

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
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相关实验视频

Updated: Jun 25, 2026

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
08:02

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 20, 2013

调节血管壁和高胆固醇贫血子单核细胞中的内皮氧化合成酶表达.

A Jiménez1, M M Arriero, A López-Blaya

  • 1Fundación Jiménez Díaz, Cardiovascular Research and Hypertension Laboratory, Madrid, Spain.

Circulation
|October 10, 2001
PubMed
概括

高胆固醇血症通过60kDa的蛋白质与eNOS mRNA结合,减少了内皮氧化合成酶 (eNOS) 的表达. 在子中,瑞瓦斯塔丁治疗逆转了这些影响,改善了内皮功能.

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LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
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相关实验视频

Last Updated: Jun 25, 2026

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
08:02

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 20, 2013

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
07:29

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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科学领域:

  • 心血管生物学 心血管生物学
  • 分子医学是分子医学.
  • 内皮细胞功能 内皮细胞功能

背景情况:

  • 培养的内皮细胞显示出细胞质蛋白破坏内皮氧化合成酶 (eNOS) 的稳定. mRNA.
  • 高胆固醇血症是内皮功能障碍的一个模型.

研究的目的:

  • 在高胆固醇贫血的子中研究eNOS mRNA结合蛋白的体内存在.
  • 确定高胆固醇血症对eNOS表达和mRNA稳定性的影响.

主要方法:

  • 在子大动脉段中评估内皮依赖放松.
  • 测量了eNOS表达和eNOS mRNA稳定性.
  • 分析了细胞质蛋白与eNOS mRNA的结合,使用西方斑点和RNA免疫沉.

主要成果:

  • 高胆固醇血症降低了内皮依赖放松和大动脉eNOS表达.
  • 一种60kDa的细胞质蛋白显示对eNOSmRNA的结合增加,从而降低了其稳定性.
  • 瑞瓦斯塔丁治疗恢复了放松,上调了eNOS,并减少了蛋白质与eNOS mRNA的结合.

结论:

  • 一种60kDa的蛋白质与eNOS mRNA结合,从而降低高胆固醇血症中eNOS的表达.
  • 这种机制影响血管壁和单核细胞.
  • 瑞瓦斯塔丁可以防止这些有害影响,突出其治疗潜力.