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相关实验视频

Updated: Jun 19, 2026

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
09:24

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

Published on: September 13, 2011

在血液透的子心脏中,心肌缺血的早期阶段,的短暂替代物的空间异质性.

Y W Qian1, W T Clusin, S F Lin

  • 1Cardiovascular Division, Stanford University School of Medicine, Stanford, CA, USA.

Circulation
|October 24, 2001
PubMed
概括

缺血期间的过渡替代体显示了哺乳动物心脏的空间异质性. 过渡的这种早期阶段空间变化可能会导致电动不稳定和心律失常.

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科学领域:

  • 心血管生理学心血管生理学
  • 心脏电生理学 心脏电生理学
  • 信号传递 信号传递

背景情况:

  • 光学映射可以使用长波长指示器可视化哺乳动物心脏中的细胞质 ([Ca2+]) 过渡物.
  • 假设在缺血期间节奏到节奏[Ca2+]的短暂交替物会导致异质的激活膜电流.

研究的目的:

  • 在哺乳动物心脏早期缺血期间调查[Ca2+]的发生和空间分布.
  • 为了确定是否因缺血引起的[Ca2+]i) 过渡替代体表现出空间和时间异质性.

主要方法:

  • 隔离的子心脏装载了光指示器rhod-2 AM.
  • 在血液透性缺血学试验中,心脏被以300/秒的速度成像.
  • 替代品的定量地图是使用替代品比率构建的.

主要成果:

  • 在所有8个测试的心脏中都成功记录了高质量的[Ca2+]过渡物.
  • [Ca2+]在所有心脏中,在控制组中缺席的短暂替代体在缺血后2-4分钟内出现.
  • 替代发生在局部区域 (5-15毫米),在整个心脏表面具有显著的空间和相异质性.

结论:

  • 缺血症在早期阶段引起[Ca2+]的明显空间异质性.
  • 过渡的这种空间异质性是电动不稳定的潜在机制.
  • 这些发现表明,早期缺血性替代体与大型哺乳动物心脏中心律不整的发展之间存在联系.

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Last Updated: Jun 19, 2026

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
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Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart

Published on: September 13, 2011

Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
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Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart

Published on: September 10, 2015

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