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Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
螺旋式金属复合体通过修改的环极素 (cyclodextrins) 进行奇拉识别
1Department of Molecular Science and Technology, Faculty of Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan.
Journal of the American Chemical Society
|October 25, 2001
概括
像鲁丁 (II) (Tris) (1,10-phenanthroline) 这样的奇拉金属复合物被修改的环氧素识别出来. 特定的环极素,heptakis(6-carboxymethylthio-6-deoxy) -beta-cyclodextrin和hexakis(2,3,6-tri-O-methyl) -alpha-cyclodextrin,表现出对抗选择性结合,这表明它们具有性识别能力.
科学领域:
- 协调化学 协调化学
- 超分子化学 超分子化学
- 基拉尔识别 基拉尔识别 基拉尔识别
背景情况:
- 奇拉性是化学和生物学的一个基本性质.
- 环极素在超分子化学中被广泛用作宿主分子.
- 金属复合物,特别是具有性结构的金属复合物,在各种应用中都很重要.
研究的目的:
- 为了研究金属复合物M(phen) 3(n+) 的奇拉性识别,通过修饰的环极素.
- 为了确定结合常数和热力学参数,以复杂化等离子体.
- 阐明选性结合背后的机制和驱动力.
主要方法:
- 谱光度学定位以确定约束常数 (K).
- 异热定位热量计 (ITC) 用于研究热力学参数 (和变化).
- 在pD 7.0下使用D2O溶剂和酸盐缓冲器进行实验.
主要成果:
- 赫普塔基斯 (heptakis) ((6-carboxymethylthio-6-deoxy) -β-cyclodextrin (per-CO2(-) -β-CD) 显示了与德尔塔- (Delta-Ruphen) 3 2+) 的优先结合.
- 赫萨克斯的2,3,6-三-O-甲基) -阿尔法-环氧德素 (TMe-α-CD) 呈现出对Ru () 3 () 2+和Ru () bpy () 3 () 2+的反选择性结合.
- 对于Fe (II),Co (II) 和Zn (II) 复合体,观察到具有per-CO2 (II) -β-CD的Pfeiffer效应,这表明有性诱导.
结论:
- 修改后的环极素可以有效地识别和区分奇拉金属复合物的反体.
- 结合机制涉及库伦相互作用的组合,纳入宿主腔,和范德瓦尔斯力.
- 具体的相互作用受到客分子的性和环极素宿主的结构特征的影响.
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