多种耐药沙门氏菌的完整基因组序列 Typhi CT18 enterica 血清
J Parkhill1, G Dougan, K D James
1The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. parkhill@sanger.ac.uk
Nature
|October 26, 2001
概括
沙门氏菌Typhi (S. typhi) 导致伤寒热. 基因组测序揭示了基因退化和耐药性,这可能解释了它对人类的特定感染和毒性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 沙门氏菌 (Salmonella enterica serovar Typhi,S. typhi) 是伤寒的致病原体,是全球严重的健康问题,每年有数百万例病例和数十万例死亡.
- 虽然许多S. enterica血清菌侵入肠道粘膜,但S. typhi独特地扩散到更深层的人体组织,如肝脏,脏和骨髓.
- 了解S. typhi宿主特异性和入侵能力的遗传基础对于开发有效的治疗方法和控制策略至关重要.
研究的目的:
- 测序和分析一种耐多药沙门氏菌Typhi (CT18) 菌株的基因组.
- 确定导致S. typhi受人类限制的宿主范围和入侵潜力的遗传特征.
- 调查与S. typhi CT18相关的等离子体的遗传组成,包括那些赋予耐药性的等离子体.
主要方法:
- 沙门氏菌Typhi CT18菌株的全基因组测序.
- 对大肠杆菌进行比较基因组分析,以确定插入,删除和伪基因.
- 血测序和pHCM1 (耐药性) 和pHCM2 (密码性) 的表征.
主要成果:
- 与大肠杆菌相比,4.8 Mbp的S. typhi CT18基因组含有大量的插入和删除.
- 鉴定了200多种伪基因,其中一些与萨尔摩内拉型菌的已知毒性基因相对应,这表明基因降解.
- CT18含有大型多药耐药性等离子体 (pHCM1) 和密码性等离子体 (pHCM2),与Yersinia pestis有联系.
结论:
- 由伪基因形成证明的遗传退化可能有助于S. typhi适应人类特异性宿主.
- 存在特定的等离子体,特别是赋予pHCM1的耐药性,是这种S. typhi菌株的一个关键特征.
- 比较基因组学提供了对宿主特异性和S. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi. typhi.
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