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萨尔门氏菌 (Salmonella enterica) 血清菌Typhimurium LT2 的完整基因组序列
M McClelland1, K E Sanderson, J Spieth
1Sidney Kimmel Cancer Center, 10835 Altman Row, San Diego, California 92121, USA. mmcclelland@skcc.org
Nature
|October 26, 2001
概括
沙门氏菌Typhimurium (S. typhimurium) 的基因组测序揭示了细菌之间频繁的基因转移. 这项研究确定了特定的S. typhimurium基因,这些基因对于了解细菌病原体和开发新疗法至关重要.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 细菌病原体的产生
背景情况:
- 沙门氏菌 (Salmonella enterica) 亚种I,血清型Typhimurium (S. typhimurium) 导致人类的胃肠炎,是伤寒发烧的模型.
- 全球非伤寒型沙门氏菌的发病率正在上升,导致人类显著的发病率和死亡率.
研究的目的:
- 为了测序S. typhimurium LT2基因组和毒性等离子体.
- 分析相关肠杆菌中的基因分布和侧向基因转移.
- 为了确定S. typhimurium亚种I-特定基因的流行病学和治疗应用.
主要方法:
- 对S. typhimurium LT2染色体和等离子体进行全基因组测序.
- 使用现有和新测序的基因组 (S. paratyphi A,K. pneumoniae) 的比较基因组学.
- 微阵列杂交以分析未测序的肠细菌中的基因存在.
主要成果:
- 测序了S. typhimurium LT2基因组 (4.857 Mb) 和等离子体 (94 kb).
- 观察到频繁的横向基因转移,与S. enterica serovar Typhi和Escherichia coli K12相比,存在显著的基因差异.
- 确定了352个S. typhimurium亚种I特定基因,其中50个可能可出口.
结论:
- 对比基因组学为S. typhimurium进化和宿主特异性提供了洞察力.
- 亚种I特异性基因代表了针对沙门氏菌感染的新型疫苗和治疗方法的潜在目标.
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