相关实验视频
Updated: Jul 6, 2026

06:28
Sample Preparation for Mass Cytometry Analysis
Published on: April 29, 2017
对于多能干细胞来说,这是开始的结束
1Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA. pdonovan@lac.jci.tju.edu
Nature
|November 2, 2001
概括
多能干细胞为组织修复和理解胚胎发育提供了强大的资源. 这些适应性细胞可以无限期培养,同时保持它们形成任何细胞类型的潜力.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 再生医学是一种再生医学.
背景情况:
- 多能干细胞具有独特的能力,在培养中无限期自我更新.
- 这些细胞在广泛扩张期间保持正常的型.
- 它们有潜力分化为体内的任何细胞类型.
研究的目的:
- 突出多能干细胞在再生医学中的潜力.
- 强调它们在研究人类胚胎发育中的有用性.
- 为了强调它们产生多样化的细胞系的能力.
主要方法:
- 长期扩张的细胞培养技术.
- 型测定用于评估染色体稳定性.
- 差异化研究,以评估发展潜力.
主要成果:
- 证明了多能干细胞的无限期扩张.
- 在整个培养过程中证实了正常型的维持.
- 展示了产生各种专门细胞类型的潜力.
结论:
- 多能干细胞是组织修复策略的宝贵资源.
- 这些细胞为研究人类早期发育提供了一个独特的模型.
- 它们的广泛差异化能力支持在再生疗法中的应用.
相关概念视频
What is the Cell Cycle?
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
Maturation of Endosomes
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Non-equilibrium in the Cell
An important concept in studying metabolism and energy is that of chemical equilibrium. Most chemical reactions are reversible. They can proceed in both directions, releasing energy into their environment in one direction, and absorbing it from the environment in the other direction. The same is true for the chemical reactions involved in cell metabolism, such as the breaking down and building up of proteins into and from individual amino acids, respectively. Reactants within a closed system...
What is the Cell Cycle?
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

