相关实验视频

Updated: May 30, 2026

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
10:51

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Published on: December 5, 2016

对温度敏感的细胞循环突变:一种中国仓鼠细胞系,具有可逆阻断的细胞动力学

J Hatzfeld, G Buttin

    Cell
    |June 1, 1975
    PubMed
    概括

    开发出一种热敏细胞系 (TS 111) 在39°C时阻断细胞运动,从而产生多核巨细胞. 在回归34°C时,细胞分裂恢复,与细胞素B效应有相似之处.

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    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols12:02

    Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

    Published on: June 6, 2017

    Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis08:33

    Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

    Published on: December 5, 2017

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    Negative Regulator Molecules01:23

    Negative Regulator Molecules

    Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
    Inhibition of Cdk Activity02:34

    Inhibition of Cdk Activity

    The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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    Replicative Cell Senescence

    Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
    Separation of Sister Chromatids02:17

    Separation of Sister Chromatids

    At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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    Meiosis II02:02

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    Molecular Factors Affecting Cell Division

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