特里托拉克斯和dCBP在一个复合体中作用,以维持同源基因的表达
1Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.
概括
含有Trx,dCBP和Sbf1的三甲基乙化复合体 (TAC1) 维持了霍克斯基因表达. 这种复合物乙化基因组,对表观遗传基因活动维护至关重要.
科学领域:
- 发育生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子生物学分子生物学
背景情况:
- 特里索拉克斯 (Trx) 是一个关键的表观遗传调节器,在发育过程中保持活跃的霍克斯基因表达.
- 三胸组 (trxG) 包含对发育基因调节至关重要的蛋白质.
研究的目的:
- 为了研究Tritorax相关复合物的功能和组成.
- 阐明特里索拉克斯乙化复合体 (TAC1) 在维持霍克斯基因表达中的作用.
主要方法:
- 从Drosophila胚胎中净化三甲基乙化复合体 (TAC1).
- 染色体免疫沉 (ChIP) 用于识别聚烯染色体上的蛋白质结合部位.
- 对trx和dCBP突变的反应中基因表达的分析.
主要成果:
- 从Drosophila胚胎中净化的TAC1含有Trx,dCBP和Sbf1.1,它们的含量很高.
- TAC1表现出类似于CBP的氨酸转移酶活性,类似于CBP.
- dCBP和Sbf1在特定染色体位点与Trx同位,包括Ultrabithorax (Ubx) 基因位点.
- 在trx或dCBP的突变减少Ubx基因表达.
结论:
- 特里索拉克斯,dCBP和Sbf1形成一个功能复合体 (TAC1),参与维持活跃的霍克斯基因表达.
- 通过TAC1对基因活性的表观遗传维护可能很重要.
- 这些蛋白质在发育过程中在调节霍克斯基因表达方面具有物理和功能联系.
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