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相关概念视频

Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

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The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
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Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

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Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
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DNA Packaging00:58

DNA Packaging

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Overview
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Polytene Chromosomes02:04

Polytene Chromosomes

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Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
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Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

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The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
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Diversity of Protists IV01:27

Diversity of Protists IV

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Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
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Signaling pathways controlling cell polarity and chemotaxis.

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相关实验视频

Updated: May 5, 2026

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging
06:09

Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging

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细胞粘液模具Dictyostelium discoideum基因组的结构组织:重复性和单复制性DNA序列的插曲.

R A Firtel, K Kindle

    Cell
    |August 1, 1975
    PubMed
    概括

    迪克托斯 DNA 包含与单复制DNA 相关的间隔重复序列. 单复制和重复的DNA序列都表现出特定的长度分布和间隙模式.

    科学领域:

    • 分子生物学分子生物学
    • 基因组学就是基因组学.
    • 发展生物学 发展生物学

    背景情况:

    • 了解基因组组织对于破译基因调节和生物体发育至关重要.
    • 狄氏基因组 (Dictyostelium discoideum) 作为研究真核生物基因表达和分化的一个模型系统.

    研究的目的:

    • 为了研究Dictyostelium中重复和单复制DNA序列的长度和间隙模式.
    • 为了确定单复制序列与间隔重复DNA相关的程度.

    主要方法:

    • 用技术对DNA片段长度进行分析,以区分单复制和重复序列.
    • 检查基因组内的不同DNA序列类型的空间布局和联系.

    主要成果:

    • 在1500个核酸片段中,大约50-60%的单复制序列和在3000个核酸片段中,大约75%的单复制序列与短暂的间隔重复有关.
    • 与重复相关的单复制序列的平均长度为1500个核酸.
    • 重复的DNA序列显示了双模长度分布,其中一半是碎片>2000个核酸,另一半是短片段 (250-450个核酸),间隔着单复制DNA.

    结论:

    • 迪克托斯 DNA 组织的特点是重复元素与单复制序列的显著间隙.

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    Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
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  • 这些发现为Dictyostelium基因组的结构组织和潜在的监管影响提供了洞察力.