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相关概念视频

Replication in Prokaryotes02:35

Replication in Prokaryotes

Overview
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Replication in Prokaryotes01:32

Replication in Prokaryotes

DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

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相关实验视频

Updated: Jul 15, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

在细菌复制叉上的两个必不可少的DNA聚合酶.

E Dervyn1, C Suski, R Daniel

  • 1Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, 78352 Cedex, France.

Science (New York, N.Y.)
|November 27, 2001
PubMed
概括

细菌细菌利用两个必不可少的DNA聚合酶进行复制,而不是一个. 新发现的dnaE(BS) 基因与polC一起,对细菌DNA合成和细胞活力至关重要.

科学领域:

  • 微生物学 微生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 细菌DNA复制传统上涉及单一的基本DNA聚合酶,以大肠杆菌中的dnaE和B. subtilis中的polC为例.
  • 在B. subtilis中发现的dnaE ((BS),与大肠杆菌的dnaE同源,促使进一步调查其作用.

研究的目的:

  • 调查dnaE(BS) 基因在Bacillus subtilis DNA复制中的基本性和功能.
  • 为了确定B. subtilis是否在复制分叉处拥有多个基本DNA聚合酶.

主要方法:

  • 细菌遗传学和基因组分析.
  • 评估B. subtilis的细胞活力和DNA复制延长.
  • 研究dnaE (BS) 在滞后链合成中的作用及其与复制工厂的联系.

主要成果:

  • 在B. subtilis中,dnaE(BS) 基因对于细胞活力和DNA复制延长至关重要,类似于polC.
  • B. subtilis在其复制分叉中拥有两种不同的基本DNA聚合酶.
  • dnaE(BS) 参与滞后链合成,并与复制工厂有关.

结论:

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CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

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Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

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CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

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  • 细菌细菌利用两个必不可少的DNA聚合酶进行复制,这是以前在真核生物中观察到的发现.
  • 这表明一种保守的机制,其中两个聚合酶 (polC和dnaE) 处理B. subtilis和其他细菌中两个DNA链的合成.