微阵列分析和Drosophila中昼夜基因表达的组织
1Department of Biology, National Science Foundation Center for Biological Timing, Waltham, MA 02454, USA.
Cell
|December 6, 2001
概括
这项研究表明,CLOCK (CLK) 转录因子控制了Drosophila的所有昼夜基因表达,大多数基因被间接调节. 研究人员确定了134个循环基因,涉及到各种生物过程.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 时间生物学 时间生物学
背景情况:
- 昼夜节律是内生生物过程,对于调节日常生理和行为模式至关重要.
- 时钟 (CLK) 转录因子是调节昼夜节律的分子机制的关键组成部分.
- 了解CLK调节基因的全部范围对于破译昼夜网络架构至关重要.
研究的目的:
- 为了全面地绘制Drosophila melanogaster的全球昼夜基因表达.
- 为了确定CLOCK (CLK) 转录因子的直接和间接目标.
- 阐明管理昼夜基因网络的调节机制.
主要方法:
- 高密度寡核酸阵列用于全球基因表达特征分析.
- 对时钟突变 (Clk) 进行分析,以评估依赖于CLK的基因调节.
- 差异显示技术用于识别特定的基因标.
主要成果:
- 鉴定了134个参与各种生物过程的循环基因.
- 观察到许多时钟控制的基因被组织成共同调节的集群.
- 证实所有振荡基因表达在CLK控制下,没有发现独立的昼夜系统.
- 在Clk突变中,大量额外的基因受到影响,这表明Clk网络的影响更广泛.
- 鉴定了有限的一组直接的CLK标基因,这表明主要是对昼夜网络的间接调节.
结论:
- 时钟 (CLK) 转录因子是Drosophila中昼夜基因表达的主调节者.
- 昼夜基因网络在很大程度上是通过CLK间接调节的,只有少数直接目标.
- 在核心昼夜过程之外,CLK会影响遗传网络.
- 基因聚类可能在昼夜输出的协调转录调节中发挥作用.
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02:19Circadian Rhythms and Gene Regulation
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