快速杀死肺炎链球菌通过菌体细胞壁氧化酶
J M Loeffler1, D Nelson, V A Fischetti
1Laboratory of Bacterial Pathogenesis, The Rockefeller University, New York, NY 10021, USA.
概括
一种新型的酶,Pal,从鼻中快速消除肺炎链球菌,包括耐药菌株. 这种酶具有很高的特异性,并防止耐药性,为肺炎球菌携带提供了潜在的新干预措施.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 传染性疾病 传染性疾病
背景情况:
- 鼻肺炎菌的鼻运输是社区传播的重要储库.
- 目前消除肺炎球菌携带者的干预措施有限,特别是在抗生素耐药菌株方面.
研究的目的:
- 为了评估净化肺炎球菌溶酶 (Pal) 在快速消除Streptococcus pneumoniae携带的疗效.
- 评估酶的特异性和耐药性发展潜力.
主要方法:
- 在体外测试Pal酶对15种常见的肺炎 estreptococcus血清型,包括耐青素菌株.
- 使用肺炎球菌殖民的小鼠模型进行体内研究,以评估酶的有效性和对细菌标位的影响.
- 评估Pal酶对其他口腔菌微生物的影响以及对Pal耐药性肺炎球菌的评估.
主要成果:
- 帕尔酶在接触后几秒钟内迅速杀死15种常见的肺炎链球菌血清型.
- 在体内,单一的Pal酶治疗使之前殖民的小鼠在5小时后具有无法检测的肺炎球菌标位.
- 该酶对其他口微生物的影响很小,甚至没有影响,在长时间暴露后没有检测到PAL耐药性肺炎球菌.
结论:
- 纯化Pal酶是一种高效和快速的消除Streptococcus pneumoniae携带的药物.
- 帕尔酶表现出极好的特异性,向肺炎球菌而不会损害共生植物群,也不会诱导耐药性.
- 帕尔代表了一种有前途的新型治疗候选药物,通过向其储存库来控制肺炎球菌病.
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