线粒体合因子6存在于人体血管内皮细胞的表面,由剪切应力释放出来
1Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan. osanait@cc.hirosaki-u.ac.jp
Circulation
|December 19, 2001
概括
血管内皮细胞释放线粒体合因子6 (CF6),一种压力剂物质. 剪切应力显著增加了这些细胞中CF6的释放,表明其在循环中的调节.
科学领域:
- 心血管生物学 心血管生物学
- 细胞生理学 细胞生理学
- 生物化学 生物化学
背景情况:
- 线粒体合因子6 (CF6) 是前环素合成的内源性抑制剂.
- CF6在全身循环中循环,并在老鼠中充当压力剂物质.
- 研究了循环CF6的起源,专注于血管内皮细胞.
研究的目的:
- 要确定血管内皮细胞是否是循环CF6的来源.
- 为了研究流体剪切应力对内皮细胞CF6释放的影响.
主要方法:
- 使用培养的人类静脉内皮细胞 (HUVEC) 和ECV 304细胞.
- 免疫光显微镜检测与表面相关的CF6.
- 在静态和剪切应力条件下,在培养基中测量CF6度.
- 在细胞同质体和线粒体中分析了CF6含量.
- 评估了CF6与GAPDHmRNA的比率.
- 流细胞计量测量以量化与表面相关的CF6.
主要成果:
- 免疫光检测证实了ECV 304和HUVEC的表面相关CF6.
- 在静态条件下,介质中的CF6度随着时间的推移而增加.
- 流体剪切应力显著增强了CF6释放,在较高的剪切水平 (25dynes/cm2) 上,度急剧上升.
- 切割应力增加了细胞同质体和线粒体中的CF6含量,在15dynes/cm2.
- 剪切应力提高了CF6与GAPDHmRNA的比率.
- 在剪切应力暴露后,与表面相关的CF6增加.
结论:
- 血管内皮细胞是CF6的重要来源.
- 从内皮细胞中释放与表面相关的CF6是由流体剪切应力调节的.
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