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相关概念视频

Titration of a Polyprotic Acid02:08

Titration of a Polyprotic Acid

A polyprotic acid contains more than one ionizable hydrogen and undergoes a stepwise ionization process. If the acid dissociation constants of the ionizable protons differ sufficiently from each other, then the titration curve for such polyprotic acid generates a distinct equivalence point for each of its ionizable hydrogens. Therefore, titration of a diprotic acid results in the formation of two equivalence points, whereas the titration of a triprotic acid results in the formation of three...
Acid-Base Titration Curves02:23

Acid-Base Titration Curves

A titration curve is a plot of some solution property versus the amount of added titrant. For acid-base titrations, solution pH is a useful property to monitor because it varies predictably with the solution composition and, therefore, may be used to monitor the titration’s progress and detect its endpoint. Acid-base titration can be performed with a strong acid and a strong base, a strong acid and a weak base, or a strong base and a weak acid.
For a titration carried out for 25.00 mL of 0.100...
Solution Composition During Acid/Base Titrations01:17

Solution Composition During Acid/Base Titrations

The titration of a weak acid with a strong base results in the formation of water and the conjugate base of the acid. For instance, titrating acetic acid with sodium hydroxide leads to the formation of water and sodium acetate. A solution of acetic acid and sodium acetate constitutes a buffer whose relative concentration at different stages of the titration is indicated by the α values, which represent percentages of the weak acid and its conjugate base.
The α0 and α1 values represent the...
Titration of Polyprotic Base with a Strong Acid01:18

Titration of Polyprotic Base with a Strong Acid

The titration of a polyprotic base such as sodium carbonate with a strong acid such as hydrochloric acid results in two equivalence points on the titration curve. At the first equivalence point, the carbonate ions in the base are completely converted to bicarbonate ions. The second equivalence point corresponds to the complete conversion of bicarbonate ions to carbonic acid, which dissociates into carbon dioxide and water. The region before the first equivalence point corresponds to the...
Titration of a Weak Acid with a Strong Base01:30

Titration of a Weak Acid with a Strong Base

In titrating a weak acid with a strong base, different calculation methods are applied at various stages. Initially, the pH of a weak acid like acetic acid is calculated using its dissociation constant (Ka) and an ICE table. Upon addition of a strong base such as sodium hydroxide, a buffer forms, and its pH is determined using the Henderson-Hasselbalch equation. As more base is added and the titration reaches the halfway point, the pH becomes equal to the pKa of the acid, indicating equal...
Titration of Polyprotic Acids with a Strong Base01:23

Titration of Polyprotic Acids with a Strong Base

Titration of a polyprotic acid, which contains multiple ionizable protons, involves distinct dissociation steps, each with its own dissociation constant (Ka). Each successive Ka is weaker than the previous one. In the titration of a polyprotic acid like sulfurous acid with a strong base such as sodium hydroxide, the base first neutralizes the initial ionizable proton, forming an intermediate species (e.g., hydrogen sulfite ions). This step's titration curve resembles that of a weak monoprotic...

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相关实验视频

Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

多站点酸化和S阶段的倒计时

R J Deshaies1, J E Ferrell

  • 1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA. deshaies@its.caltech.edu

Cell
|January 10, 2002
PubMed
概括
此摘要是机器生成的。

SCF ((Cdc4) 泛基因酶特别针对Sic1的六个酸盐,而不是五个. 这一发现揭示了一个分子计数机制,将模拟信号转换为蛋白质纳米处理器的数字输出.

更多相关视频

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
10:27

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

Published on: October 5, 2017

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

相关实验视频

Last Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
15:19

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte
10:27

Simultaneous Multi-surface Anodizations and Stair-like Reverse Biases Detachment of Anodic Aluminum Oxides in Sulfuric and Oxalic Acid Electrolyte

Published on: October 5, 2017

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
12:49

Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry

Published on: April 4, 2018

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 细胞调节 细胞调节 细胞调节

背景情况:

  • Sic1是一种通过酸化调节的细胞循环抑制剂.
  • SCF ((Cdc4) 是一种E3泛基因酶,参与细胞循环进展.
  • 酸化水平通常决定蛋白质的功能和降解.

研究的目的:

  • 调查SCF ((Cdc4) 认可的Sic1无处不在的精确酸化值.
  • 阐明SCF(Cdc4) 区分Sic1的不同酸化状态的机制.
  • 了解在生物环境中,模拟酸化信号如何转化为数字输出.

主要方法:

  • 在试验室结合试验中使用纯化的SCF (Cdc4) 和Sic1变体,具有定义的酸化状态.
  • 乌比基化试验用于监测SCF的Sic1修饰 (Cdc4).
  • 质谱测量以确认酸化位点,并对Sic1.1进行石化测量.

主要成果:

  • SCF ((Cdc4) 在六个位点选择性地结合并无处不在化Sic1,但不是五个位点.
  • 这种依赖酸化的无处不在会触发Sic1降解.
  • 该研究表明,基于"模拟"输入 (酸盐数量) 的"数字"开关.

结论:

  • SCF ((Cdc4) 作为分子计数器,利用精确的酸化值进行基质识别.
  • 这种机制从模拟输入 (不同酸化水平) 提供数字输出 (无处不在/降解).
  • 了解这种蛋白质纳米处理器架构,可以了解精确的细胞调节和信号传导.