在多种发育过程中,类缩蛋白同源体作为转录性核心压缩剂起作用
Sheng Zhang1, Lei Xu, Janet Lee
1Howard Hughes Medical Institute, Department of Genetics, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06535, USA.
Cell
|January 17, 2002
概括
牙骨-骨形缩与阿托芬-1蛋白有关. 这项研究揭示了氨酸蛋白作为转录核心抑制剂,这表明转录放松调节有助于神经退行.
科学领域:
- 分子生物学分子生物学
- 神经科学是一个神经科学.
- 遗传学 是一个遗传学.
背景情况:
- 牙骨骨化性缩 (DRPLA) 是一种神经退行性疾病,与阿托芬-1蛋白中的多重质胺扩张有关.
- 在体内Atrophin-1的功能仍然在很大程度上是未知的,阻碍了对DRPLA病变的理解.
研究的目的:
- 为了研究氨酸蛋白的体内功能.
- 探索氨酸在转录调节中的作用.
- 阐明氨酸功能障碍对神经退行性疾病的潜在贡献.
主要方法:
- 编码阿特罗芬家族蛋白质的多索菲拉基因的表征.
- 对Drosophila的突变表型的分析.
- 在体内和体外结合测定Drosophila Atrophin和Even-skipped之间的结合.
- 在体内转录抑制试验中使用人类的Atrophin-1和Drosophila Atrophin.
主要成果:
- 德洛索菲拉 (Drosophila Atrophin) 对于胚胎模式是必不可少的,并且在遗传上与转录抑制剂相互作用.
- 德洛索菲拉阿特罗芬在试验室中直接与Even-skipped结合.
- 人类的阿特罗芬-1和多索菲拉的阿特罗芬都在体内作为转录抑制剂发挥作用.
- 在Atrophin-1中的多重胺扩张减少了其转录抑制活性.
结论:
- 氨酸蛋白质作为多功能转录核心压缩剂起作用.
- 通过阿托芬调节的转录过程的失调可能会导致像DRPLA这样的神经退行性疾病的发病.
相关概念视频
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...