关于水与生物大分子相互作用的不弹性不连贯中子散射研究
Stuart V Ruffle1, Ilias Michalarias, Ji-Chen Li
1Department of Biomolecular Sciences, UMIST, P.O. Box 88, Manchester M60 1QD, UK.
Journal of the American Chemical Society
|January 24, 2002
概括
靠近DNA和膜的细胞水与散装水的行为不同,显示出不同的能量传输光谱. 这表明,细胞水的很大一部分是独特地围绕生物巨分子结构.
科学领域:
- 生物物理学的生物物理.
- 结构生物学 结构生物学
- 物理化学 物理化学
背景情况:
- 水 - 大分子相互作用对生物过程至关重要.
- 了解细胞水的结构是理解生物分子功能的关键.
研究的目的:
- 为了研究DNA和蛋白脂膜的水分状态.
- 描述水与生物巨分子相互作用的特性.
主要方法:
- 使用了不弹性不连贯的中子散射 (IINS).
- 研究对水-DNA和水-蛋白质脂质膜系统进行了研究,涉及各种水化水平.
主要成果:
- 观察到一个明显的过渡点,区分散装水和结合水.
- 与DNA和膜相互作用的水与散装水相比显示出改变的能量传递光谱.
- 估计了DNA和膜周围扰乱的水的数量.
结论:
- 细胞水的很大一部分存在于不同于散装水的状态.
- 接近生物大分子的水的结构和行为不寻常.
- 靠近的水表现出与散装溶剂不同的特性,影响生物功能.
相关概念视频
01:24Van der Waals Interactions
Atoms and molecules interact with each other through intermolecular forces. These electrostatic forces arise from attractive or repulsive interactions between particles with permanent, partial, or temporary charges. The intermolecular forces between neutral atoms and molecules are ion–dipole, dipole–dipole, and dispersion forces, collectively known as van der Waals forces.Polar molecules have a partial positive charge on one end and a partial negative charge on the other end of the molecule,...
03:13Intermolecular Forces
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02:35Noncovalent Attractions in Biomolecules
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02:35Noncovalent Attractions in Biomolecules
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...