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在冠状动脉收缩期间,线粒体偏好短链脂肪酸氧化

E Douglas Lewandowski¹, Raymond K Kudej, Lawrence T White

¹Program in Integrative Cardiac Metabolism, Department of Physiology, University of Illinois at Chicago, USA. dougl@uic.edu

|January 24, 2002

概括

在低透的心肌中,短链脂肪酸 (SCFA) 优先氧化而不是长链脂肪酸 (LCFA). 这表明线粒体的氧化能力超过LCFA输入,这表明非氧信号调节脂肪酸的使用.

科学领域:

心血管生理学心血管生理学
线粒体的新陈代谢
生物化学生物化学

背景情况:

低透心肌中脂肪酸氧化的减少传统上归因于线粒体功能障碍.
这项研究研究了一种替代机制:氧化能力可能超过受调节的长链脂肪酸 (LCFA) 条目.

研究的目的:

在冠状动脉狭窄期间检查心肌脂肪酸氧化和葡萄糖代谢.
为了阐明脂肪酸利用在心脏组织的调节机制.

主要方法:

开放胸部麻醉的猪接受了左前下垂冠状动脉输液,输入了[2-(13) C]丁酸盐 (短链脂肪酸,SCFA) 和[2-(13) C]葡萄糖.
测量是在非缺血性对照中进行的,并在5小时的LAD流量减少 (40%) 后进行测量.
用同位素标记物评估SCFA和葡萄糖的心肌氧化,同时测量心室壁加厚和外腔血流.

主要成果:

左右心室收缩使左心室壁的厚度降低了45%.
低透的心肌偏好氧化SCFA而不是内源LCFA,SCFA在缺血和正常组织中对氧化有显著的贡献 (63%对38-40%).
即使保留了上心流,但降低了内心流,SCFA氧化也增加了,并且没有观察到显著的葡萄糖氧化.

结论:

线粒体氧化脂肪酸的能力超过LCFA在LAD收缩期间的LCFA进入,因为SCFA绕过LCFA线粒体运输.
观测到的代谢变化是独立于外壁组织输液的.
这些发现表明,除了氧气可用性之外,其他因素也会调节心肌低 perfusion 期间脂肪酸利用率.

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