在实验室中,疟疾寄生虫蚊子阶段的完整发展
Ebtesam M Al-Olayan1, Annette L Beetsma, Geoff A Butcher
1Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Staffordshire ST5 5BG, UK.
Science (New York, N.Y.)
|January 26, 2002
概括
研究人员开发了一种用于培养疟疾寄生虫的新体外方法,实现了传染性杂虫虫的高效生产. 这一突破使得能够在活体宿主之外研究完整的Plasmodium berghei生命周期.
科学领域:
- 疟疾学 疟疾学
- 寄生虫学的寄生虫学
- 无脊椎动物的细胞培养
背景情况:
- 疟疾寄生虫的蚊子阶段的可复制的体外培养,产生传染性杂虫,已经是一个重要的挑战超过四十年.
- 开发这样一个系统对于疟疾研究,疫苗开发和药物发现至关重要.
研究的目的:
- 建立一个可复制的体外系统,以使蚊子阶段的Plasmodium berghei.完全发育.
- 为了在体外实现高效的传染性Plasmodium berghei杂虫的生产.
主要方法:
- 培养Plasmodium berghei体细胞通过传染性杂虫.
- 使用共同培养的Drosophila S2细胞,地下膜基质和昆虫组织培养介质来培养细胞外卵囊.
- 补充培养基的巴拉氨基酸,以促进杂虫的生产.
主要成果:
- 实现了有效的Plasmodium berghei体球细胞在体外发育成传染性杂虫,与体内效率相比.
- 在不需要唾液腺入侵的情况下证明了成功的细胞外卵囊发育.
- 证实了para-aminobenzoic酸在脊髓细胞形成中的重要作用.
结论:
- 开发的体外系统允许培养和研究Plasmodium berghei的完整生命周期.
- 这种方法为推进疟疾研究提供了有价值的工具,有可能加速新干预措施的开发.
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