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Updated: Jul 29, 2026

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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通过使用固态NMR光谱学从脂质中探测 (1) H自旋扩散的膜蛋白拓学

Daniel Huster1, Xiaolan Yao, Mei Hong

  • 1Department of Chemistry, Iowa State University, Gilman Hall 0108, Ames, Iowa 50011, USA.

Journal of the American Chemical Society
|January 31, 2002
PubMed
概括

这项研究引入了一种新的固态NMR方法来确定膜蛋白结构. 该技术使用质子旋转扩散来映射嵌入脂质双层内的蛋白质区域,有助于拓确定.

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Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...