分离酶,波罗激酶,激光蛋白Slk19和Spo12在一个网络中起作用,该网络控制了在早期亚纳相期间的Cdc14局部化
Frank Stegmeier1, Rosella Visintin, Angelika Amon
1Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Cell
|February 8, 2002
概括
恐惧网络启动Cdc14释放从它的抑制剂在芽酵母早期的亚纳相. 这种早期释放随后刺激了线粒体退出网络 (MEN) 持续的Cdc14活动.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- Cdc14酸酶是发芽酵母中线性退出的关键调节者.
- 在核中,Cdc14被其抑制剂Cfi1/Net1隔离,直到发生相.
- 线粒体退出网络 (MEN) 的信号级联控制Cdc14的释放.
研究的目的:
- 确定和描述一个控制早期Cdc14释放的新型监管网络.
- 阐明 FEAR 网络与 MEN 途径之间的相互作用.
主要方法:
- 在芽酵母 (Saccharomyces cerevisiae) 中进行遗传分析.
- 研究细胞循环中的蛋白质相互作用和调节作用.
- 专注于关键的蛋白质:Cdc14,Cfi1/Net1,Cdc5,Esp1,Slk19,Spo12. 这些蛋白质是最重要的.
主要成果:
- 标识FEAR (Cdc十四早期无相释放) 网络,包括Cdc5,Esp1,Slk19和Spo12.
- 证明 FEAR 网络在早期的异位阶段从 Cfi1/Net1 中促进 Cdc14 的释放.
- 证据表明,MEN途径在晚期亚纳相期间保持Cdc14释放.
结论:
- FEAR网络在 MEN 的上游行动,启动 Cdc14 的释放.
- 由FEAR网络释放的Cdc14可能会刺激MEN活动,创建一个积极的反循环.
- 这种协调的行动确保了及时和规范的脱离线粒分裂.
相关概念视频
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At the onset of anaphase, separase, a proteolytic enzyme, is...
02:19The Spindle Assembly Checkpoint
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00:50Anaphase Promoting Complex
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02:17Separation of Sister Chromatids
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
02:19The Spindle Assembly Checkpoint
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...