内皮原生细胞血管内皮生长因子基因转移用于血管再生
Hideki Iwaguro1, Jun-ichi Yamaguchi, Christoph Kalka
1Division of Cardiovascular Research and Medicine, St Elizabeth's Medical Center, Tufts University School of Medicine, Boston, Mass 02135, USA.
Circulation
|February 13, 2002
概括
基因转移增强了内皮原生细胞 (EPC),以促进血管生长. 修改后的EPC在动物模型中改善了血液流动并减少了四肢损伤,需要较低的细胞剂量才能产生治疗效果.
科学领域:
- 再生医学是一种再生医学.
- 血管生物学 血管生物学
- 基因治疗 基因治疗
背景情况:
- 内皮原生细胞 (EPC) 在血液中循环.
- 研究调节EPC功能的基因转移.
研究的目的:
- 评估基因转移是否可以增强EPC用于治疗性新血管化.
- 在四肢缺血模型中评估VEGF修饰EPC的疗效.
主要方法:
- 在实验室中将VEGF 164的基因转移到小鼠EPC中.
- 评估EPC的扩散,粘附和融入内皮单层.
- 在体内给予VEGF转化EPCs给后肢缺血的小鼠.
主要成果:
- VEGF基因转移增加了EPC的扩散,粘附和结合.
- 转化EPCs改善了缺血四肢的新血管化和血液流量恢复.
- 与之前的研究相比,肢体缩减少了63.7%,细胞剂量明显降低.
结论:
- 在体外,VEGF基因转移有效地增强了EPC功能.
- 基因改造的EPCs代表了增强缺血性疾病中新血管化的有前途战略.
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